Part:BBa_K1362413:Design
Gp41-1 C-Intein cloning piece
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 28
Design Notes
This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.
Source
Part information and data come from Schmidt et al. [1]. The sequence was acquired from Pietrokovski et al. [2] (to be found in the supplementary data [3]) Part DNA was obtained by DNA synthesis.
References
[1] 1. Carvajal-Vallejos, P., Pallissé, R., Mootz, H. D. & Schmidt, S. R. Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J. Biol. Chem. 287, 28686–96 (2012).
[2] Bareket Dassa, Nir London, Barry L. Stoddard, Ora Schueler-Furman, Shmuel Pietrokovski Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family Nucleic Acids Res. 2009 May; 37(8): 2560–2573. Published online 2009 March 5. doi: 10.1093/nar/gkp095
[3] http://nar.oxfordjournals.org/content/suppl/2009/02/25/gkp095.DC1/nar-00060-s-2009-File008.pdf